This laboratory is interested in two major research areas: large-scale cloning and analyses of genes involved in early cotton fiber development, and tuberculosis DNA vaccine development. Using cDNA prepared from 10dpa (days post anthesis) wild-type cotton fiber as tester and cDNA from a fuzzless-lintless (fl) mutant as driver, we applied the RDA method to recover 280 independent cDNA fragments related directly to early cotton fiber development. cDNA microarrays showed that 172 genes were significantly up-regulated in elongating cotton fibers as confirmed by in situ hybridization in representative cases. Twenty-nine cDNAs, including a putative vacuolar (H+)-ATPase catalytic subunit, a kinesin-like calmodulin binding protein, several arabinogalactan proteins and key enzymes involved in long chain fatty acid biosynthesis, accumulated to greater than 50-fold in 10 dpa fiber cells when compared to that in 0 dpa ovules. Various upstream pathways, such as auxin signal transduction, the MAP kinase pathway, and profilin- and expansin-induced cell wall loosening, were also activated during the fast fiber elongation period. The lab is also involved in cloning and expressional analyses of 8 large gene families (AP2-EREBP、bHLH、C3H-TYPE2(Zn)、E2F-DP、HMG-BOX、NIN-like、PCG、TCP) encoding Arabidopsis transcription factors. We have so far obtained about 250 full-length cDNAs and are currently working on yeast expression as well as gel mobility shift assays to study their DNA binding activities. We have done some work on elucidating molecular mechanisms of plant apical senescence using G2 pea as a model system. We showed that transgenic Arabidopsis plants carrying a putative chloroplast membrane-localized calcium channel protein (the PPF1 gene) in sense orientation flowered much later compared to control plants whereas antisense expression of this gene rendered the recipients a very rapid life cycle. The strength of this late-flowering phenotype correlated roughly with the level of PPF1 transcripts and with the calcium storage capacity in the chloroplasts of different transgenic lines. We suggest that PPF1 is a novel calcium ion carrier that regulates flowering time of higher plants by changing the Ca2+ storage capacity within chloroplasts. We have cloned five genes encoding different secreted antigens Ag85B, MPT-83, MPT-64, MPT-63 and ESAT-6 from Mycobacterium Tuberculosis. We are currently developing either combined or polyvalent DNA vaccines with different combinations. Experimental results obtained both from mice and from cows showed that immunization of combined DNA vaccines expressing 3 or 4 secreted antigens might produce significant protection against microbial invasion. Our data indicated that antigen-specific IFN-γlevels, rather than antibody levels, might be a better criteria for protective efficacy assessment.

• 植物功能基因的克隆与研究进展.ppt
• 生物化学与分子生物学进展课程.pdf
• 分子生物学演义.pdf

• Yong-Hui Shi,Sheng-Wei Zhu,Xi-Zeng Mao,Jian-Xun Feng,Yong-Mei Qin,Liang Zhang,Jing Cheng,Li-Ping Wei,Zhi-Yong Wang,Yu-Xian Zhu,(2006)Transcriptome Profiling, Molecular Biological, and Physiological Studies Reveal a Major Role for Ethylene in Cotton Fiber Cell Elongation. The Plant Cell, Vol. 18, 651–664, March 2006.
• Feng, J.-X., Liu, D., Pan, Y., Gong, W., Ma, L., Luo, J., Deng, X. W. and Zhu, Y.-X. (2005) A systematic annotation update via cDNA sequence analysis and comprehensive profiling of developmental, hormonal or environmental responsiveness of the Arabidopsis AP2/EREBP transcription factor gene family. Plant Molecular Biology. 59, 853-868.
• Fu, Q., Wang, B C., Jin, X., Li, H B., Han, P., Wei, K H., Zhang, X M. and Zhu, Y.-X. (2005) Proteomic analysis and extensive protein identification from dry, germinating Arabidopsis seeds and young seedlings. J Biochem. Mol. Biol. 38, 650-660.
• Wei, G., Lei, J., Gong, W. and Zhu, YX. (2005) Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana. Chinese Science Bulletin. 50: 1873-1878. 魏刚,雷娟,巩威,朱玉贤。（2005）拟南芥QRAP2基因的克隆、表达、DNA结合能力及体外转录活性分析。《科学通报》，50:1863-1868。
• Wei G, Pan Y, Lei J and Zhu YX. (2005) Molecular Cloning, Phylogenetic Analysis, Expressional Profiling and In Vitro Studies of TINY2 from Arabidopsis thaliana. J Biochem. Mol. Biol. 38:440-446.
• Liu D, Gong W, Bai Y, Luo JC and Zhu YX. (2005) OsHT, a rice gene encoding for a plasma-membrane localized histidine transporter. Journal of Integrative Plant Biology. 47:92-99.
• Gong W, Sun YP, Ma LG, Pan Y, Du YL, Wang DH, Yang JY, Hu LD, Liu XF, Dong CX, Ma L, Chen YH, Yang XY, Gao Y, Zhu DM, Tan XL, Mu JY, Zhang DB, Liu YL, Dinesh-Kumar SP, LI Y, Wang XP, Gu HY, Qu LJ, Bai SN, Lu YT, Li JY, Zhao JD, Zuo JR, Huang H, Deng XW, and Zhu YX. (2004) Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Plant Physiol. 135:773-782.
• Feng JX, Ji SJ and Zhu YX. (2004) Analysis of five differentially expressed gene families in fast elongating cotton fiber. Acta Biochimica et Biophysica Sinica. 36: 51-56.
• Li J, Wang DY, Li Q, Xu YJ, Cui KM and Zhu YX. (2004) PPF1 inhibits programmed cell death in apical meristems of both G2 pea and transgenic Arabidopsis plants possibly by delaying cytosolic Ca2+ elevation. Cell Calcium. 35: 71-77.
• 白勇，巩威，刘天昀，朱玉贤。(2003) 水稻肉桂酰辅酶A还原酶基因的克隆与表达分析。《科学通报》，48:1780-1784。Bai Y, Gong W, Liu TY & Zhu YX. (2003) Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings. Chinese Science Bulletin. (2003) 48: 2221-2225.
• Ji SJ, Lu YC, Feng JX, Wei G, Li J, Shi YH, Fu Q, Liu D, Luo JC and Zhu YX. (2003) Isolation and Analyses of Genes Preferentially Expressed During Early Cotton Fiber Development by Subtractive PCR and cDNA Array. Nucleic Acids Res. 31: 2534-2543.
• Wang DY, Xu YJ, Li Q, Hao XM, Su YH, Sun FZ, Zhu YX. (2003) Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering. The Plant Journal，33: 285-292.
• 徐云剑，顾雪松，李珺，李晴，Peter J. Davies，朱玉贤。（2003）豌豆乙酰羟酸还原异构酶基因结构、蛋白活性分析及其调控机制研究。《中国科学(C辑)》，33:19-26. Xu YJ, Gu XS, Li J, Li Q, Davies P and Zhu YX (2003) Studies on gene structure, enzymatic activity and regulatory mechanism of acetohydroxy acid isomeroreductase from G2 pea. Science In China, Series C. 46: 389-398.
• Xu YJ, Wang DY, Zhu YX. (2002) Expression of the thylakoid membrane localized PPF1 in transgenic Arabidopsis affects chloroplast development. Acta Botanica Sinica, 41: 1314-1320.
• Ji SJ, Liu F, Li EQ, Zhu YX. (2002) Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not human cells. Cell research, 12: 143-150. (1.94)
• Ji SJ, Lu YC, Li J, Wei G, Liang XJ, Zhu YX. (2002) A ?-tubulin-like cDNA expressed specically in elongating cotton fibers induces longitudinal growth of fission yeast. Biochemical and Biophysical Research Communications. 296: 1245-1250.
• Lu YC, Wei G, Zhu YX. (2002) Cloning whole cellulose-synthesizing operon (ayacs Operon) from Acetobacter xylinum and transforming it into cultivated cotton plants. Acta Botanica Sinica. 44: 441-445.
• Wang DY, Hu S, Li Q, Cui KM and Zhu YX (2002) Photoperiod control of the apical bud and leaf senescence in pumpkin strain 185. Acta Botanica Sinica. 44: 55-62.
• 苏彦辉，曲红，Vincent Vachon，张杰，罗静初，Raynald Laprade， 朱玉贤（2002）苏云金芽孢杆菌Cry1Aa蛋白а4螺旋形成跨膜离子通道的昆虫毒性试验与模型预测。《中国科学(C辑)》，32: 146-152。Su YH, Qu H, VACHON Vincent, Lu JC, Zhang J, LAPRADE Raynald and Zhu YX (2002) The toxicity test and hypothetical model of Bacillus thuringiensis Cry1Aa helix4. Science In China, Series C. 45: 561-567.
• 徐华松，徐云剑，顾雪松，胡廷章，苏彦辉，朱玉贤（2001）豌豆乙酰羟酸还原异构酶基因的cDNA克隆、序列分析及其表达研究。科学通报，46: 1022-1026。Xu HS, Xu YJ, Gu XS, Hu TZ, Su YH and Zhu YX (2001) Cloning and analysis of a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea. Chinese Science Bulletin, 46: 1808-1812.
• Jiang GY, Xu YJ, Zhu XS, Su YH and Zhu YX. (2001) Prokaryotically expressed Buthus martensii Karsch insect depressant toxin has insecticidal effects. Toxicon. 39: 469-476.
• Zhu YX, James M. Tepperman, Crag Fairchild and Peter H. Quail (2000) Phytochrome B binds with greater apparent affinity than phychrome A to the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS domain of PIF3. Proc. Natl. Acad. Sci. USA 97: 13419-13424.
• 徐云剑，江国英，朱玉贤（2000）转BmKIT4基因烟草的抗虫性。植物学报，42: 732－735。Hu TZ, Xu YJ, Jiang GY and Zhu YX. (2000)　Studies on insect resistance of transgenic tobacco plants carrying the BMK IT4 gene. Acta Botanica Sinica，42: 732－735.